Exploring Antibody Internalization

Discover how pH-sensitive probe–based assays and multiplexed cytometry enable quantitative evaluation of antibody internalization across multiple cell types within a high-throughput workflow.

In this application note, you’ll learn how to:

• Perform antibody internalization assays using a no-wash, pH-sensitive probe–based method.
• Analyze multiple cell populations simultaneously through multiplexing with encoding dyes.
• Evaluate the impact of antibody isotypes and immune cell differentiation on internalization.
• Characterize antibody-drug conjugates (ADCs) for binding, internalization, and cytotoxicity.

What’s Inside the Application Note:

This application note outlines a streamlined experimental workflow, including:

• ABI assay workflow using a pH-sensitive probe for precise internalization measurement.
• Multiplexed cytometry to analyze multiple cell types within a single well.
• Case study insights on how isotype variation and cell differentiation influence internalization