MHC Class II UltimersT from ProImmune
MHC Class II Ultimers™
ProImmune's MHC Class II Ultimers™: Advanced Multimer Technology for CD4+ Antigen-Specific T Cell Detection
ProImmune specializes in the production of MHC Class II Ultimers™, a cutting-edge multimer technology designed for immunologists seeking to detect and quantify CD4+ antigen-specific T cells. These Ultimers™ feature an exceptional peptide-linked planar design and optimized fluorochrome, offering superior performance.
When ordering custom Class II Ultimers™ from ProImmune, researchers can expect quick delivery in a matter of weeks, rather than months. The company ensures stringent quality control measures and maintains low batch-to-batch variability. Importantly, the staining procedure for Class II Ultimers™ is highly compatible with that of other MHC tetramers, making the transition seamless. The staining protocols for Class II Ultimers™ closely resemble those for Class II tetramers.
Just like ProImmune's Pro5® MHC Pentamers, Class II Ultimers™ are available as both custom-made and off-the-shelf products. Custom Class II Ultimers™ can be obtained within six weeks, similar to Class I Pentamers. Notably, ProImmune's Class II Ultimer™ technology is not only advanced but also affordable, with prices comparable to existing Pentamer technology.
Enhanced Conformation and Sterics for Improved Binding
Similar to MHC Pentamers, MHC Class II Ultimers™ are arranged in a planar configuration, meticulously optimized to facilitate optimal access of T Cell Receptors (TCRs) on the cell surface to the MHC binding groove. The unique feature of Class II Ultimers™ lies in their linkage to a core structure via peptides. This individual alignment of each MHC molecule with its complementary TCR significantly enhances the binding characteristics. In contrast, standard tetramers exhibit MHC molecules that point away from the central streptavidin core, resulting in their peptide binding grooves facing different directions.
Fluorescent Labeling for Optimal Signal and Low Background
The labeling technology utilized for Class II Ultimers™ has been fine-tuned to achieve the best combination of signal strength (brightness) and minimal background (non-specific) staining. Researchers can select from R-PE and APC labeled Class II Ultimers™, each offering excellent performance in terms of signal quality and specificity.
Experience the advanced capabilities of ProImmune's MHC Class II Ultimers™, enabling precise detection and characterization of CD4+ antigen-specific T cells.
