The field of real-time, label-free protein binding analysis has historically been marked with steep learning curves. The Octet® BLI systems were the first systems to challenge this notion by providing scientists with a simple yet powerful user interface in both the system and the software. The recently released Octet® SF3 system aims to achieve the same for the field of SPR with novel injection technologies that dramatically simplify experimental design and decrease time to results.

Find the tips and tricks most applicable to your experiment. Importantly, we designed this guide such that you can skip directly to the topics you need to answer questions about any label-free biomolecular interaction analysis.

Designing and performing assays that accurately measure the binding kinetics of biomolecular interactions can seem too specialized and out of reach for many scientists. This applications guide aims to change that by serving as an easy-to-use knowledge base and reference for assay design on both BLI and SPR systems.