Reprint Of “Mixed-Mode Chromatography In Pharmaceutical And Biopharmaceutical Applications”
Authors: Kelly Zhanga, Xiaodong Liub
Abstract:
In recent years, mixed-mode chromatography (MMC) has emerged as a rapidly advancing field, driven by the development of new mixed-mode stationary phases and improved understanding of multimode interactions. MMC offers significant advantages in separating compounds that are challenging to retain or resolve using conventional reversed-phase LC methods, particularly for polar and charged molecules. With its ability to provide multiple retention modes within a single column, MMC often enhances separation methods by allowing adjustments to the mobile phase conditions. Moreover, mixed-mode media proves to be an effective tool for sample matrix cleanup in purification processes and enables sensitive detection of trace analytes. This article explores the principles of mixed-mode stationary phases and separation mechanisms, and highlights recent advancements in pharmaceutical and biopharmaceutical applications. These include the analysis and purification of counterions, small molecule drugs, impurities, formulation excipients, peptides, and proteins.
Keywords
Mixed-mode chromatography; Pharmaceutics; Biopharmaceutics
Citation: Kelly Zhanga, Xiaodong Liub Reprint Of “Mixed-Mode Chromatography In Pharmaceutical And Biopharmaceutical Applications” http://dx.doi.org/10.1016/j.jpba.2016.09.013
Received: 1 February 2016, Revised: 4 May 2016, Accepted: 5 May 2016, Available online: 16 September 2016
Copyright: © 2016 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/)
Conclusion and perspectives
Mixed-mode chromatography provides unique selectivity especially for polar and charged analytes. The multiple interaction mechanisms allow the users to adjust the mobile phase/eluent conditions to promote certain interactions for certain analytes. MMC has advanced from original “secondary interaction” to purposely designed and controlled multi-mode interactions. The new generation of mixed-mode stationary phases is more robust and diverse. The recent commercialization of mixed-mode stationary phases greatly enhanced the applications of MMC.
Acknowledgements
We thank Xuefei Sun of Thermo Fisher Scientific for helping edit some of the references, Geoffrey Yeh and Sam Yang of Genentech for reviewing the manuscript.
