Cell Line Development: Multi-Transposase Strategies for Complex Molecular Assembly and Modulated Expression

While transposase-mediated gene insertion has revolutionized high-producing cell line development (CLD), relying on a single system limits engineering potential. By deploying multiple orthogonal Leap-In transposases^® with non-overlapping integration sites, we have expanded the CLD toolkit far beyond standard biologics expression.

Drawing from recent publications and case studies, this talk demonstrates how orthogonal multi-transposase strategies maximize complex molecular assembly and advanced cell line customization. We will highlight practical approaches to fine-tuning post-translational modifications—specifically modulating glycan profiles and fucosylation—and seamlessly integrating multi-domain assemblies into stable clonal lines to address tough next-generation therapeutic challenges.

What you will learn :

• Increasing Flexibility in CHO Engineering: Multiple transposases increase the potential for CHO cell line engineering.
• Utilise Orthogonal Transposases: High expression titers in addition to engineering other cellular functions and molecular improvements.
• Maintaining cell line stability: Orthogonal Leap-In Transposases stably integrate into multiple host genome loci without interfering with one another.

Who should attend :

• Biotechnology and Pharma scientists who need high-expressing cell lines for biologics
• Protein and antibody engineers interested in CHO cell line engineering
• Bioprocess and drug substance labs who want a CRO partner for cell line development